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| Bressan,Fabiana Fernandes; Fantinato-Neto,Paulo; Andrade,Gabriella Mamede; Sangalli,Juliano Rodrigues; Sampaio,Rafael Vilar; Silveira,Juliano Coelho da; Perecin,Felipe; Meirelles,Flávio Vieira. |
| This review aim to present some clinical problems found in IVP-derived animals focusing on NT procedures and to discuss the possible role of epigenetics in such process. Also, as cell-secreted vesicles have been reported as possible regulators of important physiological reproductive processes such as folliculogenesis and fertilization, it is also presented herein a new perspective of manipulating the pre-implantation period trough effector molecules contained in such vesicles. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo development; Nuclear transfer; Epigenetics; Extra-cellular vesicles.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001001879 |
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| Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del. |
| Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308 |
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| Tetzner,Tatiane Almeida Drummond; Saraiva,Naiara Zoccal; Perecin,Felipe; Niciura,Simone Cristina Méo; Ferreira,Christina Ramires; Oliveira,Clara Slade; Garcia,Joaquim Mansano. |
| Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine embryo; In vitro culture; Ovalbumin; Protein source. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001000010 |
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